Journal: Scientific Reports
Article Title: A perfusable decellularized plant-based air-liquid-interface-on-a-chip for investigating inorganic dust aerosol exposure
doi: 10.1038/s41598-025-33930-7
Figure Lengend Snippet: Diagram of air–liquid-interface platform for dust exposure. ( A ) Stepwise experimental timeline of the decellularized plant-based ALI model and dust exposure. ( B ) 3D design of the platform, which provides an enclosed chamber for accommodating spinach leaves. ( C ) The macroscopic image of the recellularized spinach integrated into the air–liquid-interface platform. Live/Dead fluorescence images (Calcein-AM = green, EthD-1 = red) of recellularized leaves (Left and right panels correspond to images taken from different focal planes representing the apical epithelial surface and the vascular endothelial layer, respectively). ( D ) Quantitative cell viability of BEAS-2B and HUVECs analyzed using ImageJ software (NIH, USA).
Article Snippet: Cell viability within the recellularized spinach scaffolds was evaluated using a Calcein-AM/Ethidium homodimer-1 (EthD-1) Live/Dead Viability/Cytotoxicity Kit (Biotium, USA) following the manufacturer’s protocol.
Techniques: Fluorescence, Software